Coding
Ateam

Part:BBa_K1023003:Design

Designed by: Shreyans Chordia (shreyanschordia90@gmail.com)   Group: iGEM13_Wageningen_UR   (2013-09-17)

FRET based ATP biosensor cds, codon optimized for Aspergillus niger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1516
    Illegal BsaI.rc site found at 779
    Illegal SapI.rc site found at 1330


Design Notes

This Biobrick is created by synthetically constructing an Aspergillus codon-optimized (Fig. 1) version of the Ateam sensor obtained from Imamura lab. Three synthetic constructs were designed, ordered and assembled via Gibbson Assembly.

Fig. 1) Aspergillus codon-optimization table

Source

Imamura lab

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735558/] Imamura, H., et al. (2009). "Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators." PNAS 106: 15651-15656.